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dcas9 vpr plasmid  (Addgene inc)


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    Structured Review

    Addgene inc dcas9 vpr plasmid
    Dcas9 Vpr Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ef1a+flag+dcas9+vpr/lenti+EF1a-FLAG-dCas9-VPR+(Plasmid+%23114195)/pmc12181958-224-4-6
    Average 93 stars, based on 6 article reviews
    dcas9 vpr plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: The Parkinson’s disease risk gene cathepsin B promotes fibrillar alpha-synuclein clearance, lysosomal function and glucocerebrosidase activity in dopaminergic neurons
    Article Snippet: .. To generate CRISPRa and CRISPRi parental cell lines, lentivirus was used to stably transduced RPE1 cells with either pLX_311-KRAB-dCas9 [ ] (Addgene #96,918, henceforth referred to as CRISPRi) or EF1a-FLAG-dCas9-VPR [ ] (Addgene #114,195, henceforth referred to as CRISPRa) and single clones were selected and characterized to generate monoclonal parental lines stably expressing the CRISPRa and CRISPRi machinery. .. The gRNA sequences targeting our genes of interest were selected from previously published CRISPRa/i libraries [ ] (Supplemental Table 6), synthesized by IDT and cloned into pCRISPRi/a-v2 [ ] (Addgene #84,832).

    Article Title: The Parkinson’s disease risk gene cathepsin B promotes fibrillar alpha-synuclein clearance, lysosomal function and glucocerebrosidase activity in dopaminergic neurons
    Article Snippet: .. To generate CRISPRa and CRISPRi parental cell lines, lentivirus was used to stably transduced RPE1 cells with either pLX_311-KRAB-dCas9 (Addgene #96918, henceforth referred to as CRISPRi) or EF1a-FLAG-dCas9-VPR (Addgene #114195, henceforth referred to as CRISPRa) and single clones were selected and characterized to generate monoclonal parental lines stably expressing the CRISPRa and CRISPRi machinery. .. The gRNA sequences targeting our genes of interest were selected from previously published CRISPRa/i libraries (Supplemental Table 6), synthesized by IDT and cloned into pCRISPRi/a-v2 (Addgene #84832).

    Clone Assay:

    Article Title: The Parkinson’s disease risk gene cathepsin B promotes fibrillar alpha-synuclein clearance, lysosomal function and glucocerebrosidase activity in dopaminergic neurons
    Article Snippet: .. To generate CRISPRa and CRISPRi parental cell lines, lentivirus was used to stably transduced RPE1 cells with either pLX_311-KRAB-dCas9 [ ] (Addgene #96,918, henceforth referred to as CRISPRi) or EF1a-FLAG-dCas9-VPR [ ] (Addgene #114,195, henceforth referred to as CRISPRa) and single clones were selected and characterized to generate monoclonal parental lines stably expressing the CRISPRa and CRISPRi machinery. .. The gRNA sequences targeting our genes of interest were selected from previously published CRISPRa/i libraries [ ] (Supplemental Table 6), synthesized by IDT and cloned into pCRISPRi/a-v2 [ ] (Addgene #84,832).

    Article Title: The Parkinson’s disease risk gene cathepsin B promotes fibrillar alpha-synuclein clearance, lysosomal function and glucocerebrosidase activity in dopaminergic neurons
    Article Snippet: .. To generate CRISPRa and CRISPRi parental cell lines, lentivirus was used to stably transduced RPE1 cells with either pLX_311-KRAB-dCas9 (Addgene #96918, henceforth referred to as CRISPRi) or EF1a-FLAG-dCas9-VPR (Addgene #114195, henceforth referred to as CRISPRa) and single clones were selected and characterized to generate monoclonal parental lines stably expressing the CRISPRa and CRISPRi machinery. .. The gRNA sequences targeting our genes of interest were selected from previously published CRISPRa/i libraries (Supplemental Table 6), synthesized by IDT and cloned into pCRISPRi/a-v2 (Addgene #84832).

    Expressing:

    Article Title: The Parkinson’s disease risk gene cathepsin B promotes fibrillar alpha-synuclein clearance, lysosomal function and glucocerebrosidase activity in dopaminergic neurons
    Article Snippet: .. To generate CRISPRa and CRISPRi parental cell lines, lentivirus was used to stably transduced RPE1 cells with either pLX_311-KRAB-dCas9 [ ] (Addgene #96,918, henceforth referred to as CRISPRi) or EF1a-FLAG-dCas9-VPR [ ] (Addgene #114,195, henceforth referred to as CRISPRa) and single clones were selected and characterized to generate monoclonal parental lines stably expressing the CRISPRa and CRISPRi machinery. .. The gRNA sequences targeting our genes of interest were selected from previously published CRISPRa/i libraries [ ] (Supplemental Table 6), synthesized by IDT and cloned into pCRISPRi/a-v2 [ ] (Addgene #84,832).

    Article Title: The Parkinson’s disease risk gene cathepsin B promotes fibrillar alpha-synuclein clearance, lysosomal function and glucocerebrosidase activity in dopaminergic neurons
    Article Snippet: .. To generate CRISPRa and CRISPRi parental cell lines, lentivirus was used to stably transduced RPE1 cells with either pLX_311-KRAB-dCas9 (Addgene #96918, henceforth referred to as CRISPRi) or EF1a-FLAG-dCas9-VPR (Addgene #114195, henceforth referred to as CRISPRa) and single clones were selected and characterized to generate monoclonal parental lines stably expressing the CRISPRa and CRISPRi machinery. .. The gRNA sequences targeting our genes of interest were selected from previously published CRISPRa/i libraries (Supplemental Table 6), synthesized by IDT and cloned into pCRISPRi/a-v2 (Addgene #84832).



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